Eman M Khalaf
University of Guelph, Canada
Title: Taxonomic diversity of cucurbit seed associated endophytes
Biography
Biography: Eman M Khalaf
Abstract
Endophytes are potentially benefi cial microbes that live inside plants and can be utilized as biofertilizers and biocontrol
agents by promoting plant growth and/or suppressing phytopathogens. Few endophytes have previously been isolated
from the cucurbit plant family which includes economically important crops including watermelon, cucumber, cantaloupe,
pumpkin and squash. In the present study, 169 bacterial endophytes and 10 fungal endophytes were isolated from seeds of
diff erent varieties of diverse cucurbits; the most comprehensive characterization of endophytes from this plant family. 16S
rDNA and internal transcribed spacers (ITS4 and ITS1F) fi ngerprinting were used to taxonomically classify the isolated
bacteria and fungi, respectively. Phylogenetic analysis of bacterial isolates showed Bacilli was the most abundant class including
six diff erent bacterial families. Th e γ-proteobacteria and Actinobacteria were also represented in four diff erent families. Bacillus
was the core microbiota that was conserved across tested cucurbits genera. Strains of the genus Paenibacillus were exclusively
isolated from Cucumis sativus L, Cucumis melo L and Cucurbita pepo L. var pepo L. Th ree diff erent genera of lactic acid bacteria
(LAB) were recovered from C. sativus L, C. melo L., Citrullus lanatus var. lanatus and Cucurbita pepo L var. turbinata. Our
results suggest co-evolution of seed microbial consortia with their host plants. We now propose to test these novel endophytic
microbial strains for their ability to suppress important fungal and bacterial pathogens of the cucurbit family.