John Thomas
International Educator and Global Microbiologist, USA
Title: Chronic wound intervention with contour-fi tting probiotic gauze: Re-establishing the benefi cal microbial library within
Biography
Biography: John Thomas
Abstract
Chronic wounds aff ect 6.5 million patients at an annual cost of US $25 billion, focusing on a growing, aging population polarized
with antibiotic resistance. We have investigated/reported on our use of eco-friendly skin probiotics with a multi-tiered in vitro
approach integrating: 1) Disruption: Reconstruction; 2) tissue engineering via a SMarT Gauze, 3) contoured to wound 3-D dimensions,
while selecting 4) 4 pools of probiotics targeted against common wound pathogens. Th e recent emergence of the hologenomic Th eory
of co-evolution catalyzed our theme of dual citizenship and the dual role of re-establishing the skin microbiota and library as a 1)
biologic and 2) antimicrobial barrier via restorative microbiology, facilitated by selection using a unique computer supported decision
tree analysis (Bac-2-Health) www.globalbugs.com. (Probiotic Solutions), recently enhanced for wounds and aging, an interesting
intersection (Partners-4-Life). Here, we expand our wound focus, targeting the elimination of wound gauze colonization (Ping- Pong
Hypothesis) which provides a biofi lm reservoir for re-inoculation of the wound bed via planktonic metastasis, disruption, while
re-establishing the healthy skin architecture reconstruction focusing on fungal pathogenicity. Secondarily, we have characterized
features of benefi cial probiotics as featured in published literature, addressing potential designer, intelligent probiotics as therapeutic
bacteria, highlighting a benefi cial biofi lm based on literature review and our evaluations, perhaps administered simultaneously, GUT
and skin, recognizing the dual axial brain link. Our strategy is to implement the 4 Es: ecologically sound, environmentally friendly,
engineered intelligently and economically feasible.