Dr. Ikram ul Haq,
Government College University Lahore
Title: Optimization of cultural conditions for the production of endoglucanase using lab scale fermenter
Biography
Biography: Dr. Ikram ul Haq,
Abstract
Endoglucanases are one of the most significant industrial enzymes and have diverse applications. Various endoglucanases are commonly produced by archaea, bacteria, fungi, plants, and animals that differ in their mode of action. Present study was aimed to optimize different cultural conditions i.e. incubation time, media, temperature, pH and inoculum size, for maximum endoglucanase production using recombinant E.coli strain by means of lab scale fermenter. Genetically modified E.coli contained gene for endoglucanase from Thermotoga petrophila. Maximum endoglucanase activity observed was 12.56 U/mg. Fermentation kinetics was also applied and specific yield product coefficient was revealed as 3.923781/U/ml/h. Best results were obtained after 24 hours of incubation time at a temperature of 37oC and pH 7.0 when 1% inoculum was added to ZYBM9 media)