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Balogun, O.B

Department of Bioscience and Biotechnology, Babcock University Ilishan Remo University.

Title: ASSESSMENT OF METHICILLIN RSISTANCE AND TOXIN ASSOCIATED GENES IN STAPHYLOCOCCUS SPECIES ISOLATED FROM TWO SELECTED PIG FARMS IN OGUN STATE

Biography

Biography: Balogun, O.B

Abstract

Swine especially pigs have been reported to harbor methicillin-resistant Staphylococcus species and have become a source of a novel and rapidly emerging infection in humans. This study was therefore, designed to investigate methicillin resistance status, susceptibility and exfoliative toxin-encoded genes in Staphylococcus species isolated from pigs.

Hundred and fifty (150) samples consisting of 50 anal, nostril and environmental swabs were collected at Ode Remo and Sapade in Ogun state after obtaining ethical clearance. These were transferred into transport medium and transported to Microbiology laboratory of Babcock University. The samples were processed and organisms isolated following Microbiological procedures. The isolates were identified to species level by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry. The phenotypic detection of methicillin resistance and susceptibility of the isolates to selected antibiotic classes were evaluated by agar diffusion and interpreted according to CLSI, 20011. Exfoliative toxin-encoding genes (eta andetb) in the isolates were screened by Polymerase Chain Reaction (PCR). The data were analyzed by descriptive statistics (frequency).

Fifty (50) staphylococcal strains were isolated from anus (28), nostril (17) and environment (5) of which Staphylococcus sciuri(23), Staphylococcus cohnii(11), Staphylococcus piscifermentas(7), Staphylococcus carnosus(1), Staphylococcus condiment (3), Staphylococcus xylosus(2), Staphylococcus Kloosii(1), Staphylococcus pasteuri(1) and Staphylococcus succinus(1). Methicillin resistance was detected in 12 strains S. xylosus (1), S. kloosii (1), S. picifermentas (2) and S. sciuri (8) with phenotypic method while none of the strains were positive by molecular counterpart. Susceptibility to other antibiotics indicated that all the strains were resistant to ceftazidimeS. sciuri(23), S. cohnii(11), S. piscifermentas(7), S. carnosus(1), S. condimenti(3), S. xylosus(2), S. kloosii(1), S. pasteuri(1), and S. succinus(1). All the strains were declared negative for exfoliative toxin encoding genes after several trails in PCR.

Methicillin resistance is absent amoung the strains studied and the resistance patterns observed indicated that the pattern of resistance predominantly found in clinical isolates are also emerging in the animal husbandry. Hence, setting up antibiotic surveillance system is necessary to minimize this trend.

Keywords: Staphylococci, exfoliative toxin, Methicillin, Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry, Polymerase Chain Reaction