Harinatha Reddy Aswartha
Ph.D,Sri Krishnadevaraya University Anantapur
Title: Isolation, screening and antibiogram identification of Staphylococcus aureus from immune suppressed patients
Biography
Biography: Harinatha Reddy Aswartha
Abstract
S. aureus is a gram positive, facultative anaerobic bacteria and common cause of skin infections, respiratory infections, bone infections, blood infections and pneumonia on humans. S. aureus opportunistic pathogen found in the skin and nose as part of human normal flora. There are five species S. aureus, S. epidermidis, S. saprophyticus, S. haemolyticus, and S. hominis consider as potential human pathogens in this genus, but among this pathogenic bacteria S. aureus is the most problematic, causes skin, joint and blood infections in humans. In the present study the Staphylococcus aureus isolated from blood samples of immune suppressed patients. The 30 blood samples collected form immune suppressed cancer patients. The S. aureus formed yellow color colonies on Mannitol salt agar media after 24 h of incubation. Among 30 blood samples the S. aureus was identified in 5 blood samples. Staphylococcus aureus was identified based on the microscopic, biochemical and molecular characterization. Antibiogram was determined by the disk diffusion method for five selected isolates. The Methicillin Resistant Staphylococcus aureus (MRSA) was identified in the selected isolates. The mecA gene identified in the S. aureus responsible for methicillin resistance, mecA is synthesis penicillin binding protein 2A (PBP2A). Penicillin binding protein 2A allows the bacteria to resistant against beta lactum antibiotics.